Advantages and disadvantages of the isolation methods of MicroRNA from human stool. Assignments | Get Homework Help

How miRNA should preferably be isolated from human faeces is not known. A variety of methods and pre-treatments are used. This study will investigate which methods are most frequently used in recent research and discuss the advantages and disadvantages of the different methods.

I have a already written paper. I want the writer to write a discussion section about the advantages and disadvantages of the isolation methods of microRNA from human stool. It could be the time consumption, if the pretreatment affects the extraction, which isolation kit (mirvana or RNeasy miRNAs) gives pure and high yield.
I don`t want to have anything about statistics/sensitivity and specificity in this paper.

I already have sources in the paper. You can just use them or you can use one or two more sources from pubmed databse. The isolation method has to be done in human stool, not other biological materials like plasma, urine.