Genetic

CASE STUDY 2

A microbiologist discovers a new restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced. What is the most likely number of base pairs in the recognition sequence of this enzyme? (5 pts.).
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How many recombinant DNA molecules of 40 kb size would you need to clone for a species whose genome size is 30,000 kb if you want to be 90 % sure that a given sequence is represented in your genomic library? (5 pts).
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“Genetic causes for human disorders are being discovered at an unprecedented pace. Disease may be caused by direct changes in epigenetic marks, such as DNA methylation, commonly found to affect imprinted gene regulation”. (Jiang et al. 2004)

Describe a case study where epigenetics plays a major role in the etiology of a human disease (include your reference). 5pts
One paragraph and include at least two references on the same topic.

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